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  1. The Upstream Processing unit of the NBC2 global biomanufacturing curriculum provides curricular material for each step of the upstream processing of a biopharmaceutical.
  2.  
  3. TEXTBOOK CHAPTER
  4. Chapter 10: Upstream Processing

  5. Production Unit Overview

  6.  

  7. Expression Systems
  8. Anti-IL8 Monoclonal Antibody production in CHO cells

  9. ⇰  mAb Upstream Process Flow Chart

  10. ⇰   Batch Record: Anti-IL-8 mAb Production from CHO DP-12 Cells
  11. ⇰   Batch Culture of anti-IL-8 Monoclonal Antibody secreting CHO DP-12 Cells SOP
  12. ⇰   Applikon ez-Control Bioreactor Operation SOP
  13. ⇰   Operation of Life Technologies Countess II Cell Counter SOP
  14. ⇰   Bellco Spinner Flask Cleaning and Autoclaving SOP 
  15. ⇰   Glucose and Lactate Concentration Determination using the YSI 2900 Biochemistry Analyzer SOP
  16. ⇰   Glucose Determination Assay Using Spectrophotometry SOP
  17. ⇰   Lactate Determination Assay Using Spectrophotometry SOP
  18. ⇰   Trypan Blue Assay for Cell Viability Determination SOP
  19.  
  20. Tissue Plasminogen Activator, tPA, production in CHO cells
  21. ⇰   Entire CHO Cell tPA Core Production System Manual  
  22. ⇰   Batch Record: tPA Production from CHO Cells Upstream Process
  23. ⇰   Batch Culture of Recombinant tPA Secreting CHO Cells SOP
  24.  
  25. Human Serum Albumin, HSA, production in Pichia pastoris cells
  26. ⇰   Entire Pichia pastoris HSA Core Production System Manual
  27. ⇰   Batch Record: HSA Production from Pichia pastoris Upstream Processing 
  28. ⇰   Process Controlled Fed-Batch Fermentation of Recombinant HSA Secreting Pichia pastoris SOP
  29.  
  30. Green Fluorescent Protein, GFP, production in Escherichia coli cells
  31. ⇰   Entire Escherichia coli GFP Core Production System Manual
  32. ⇰   Batch Record: GFP Production from E. coli Upstream Processing
  33. ⇰   Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP
  34.  
  35. Associated Upstream Processing SOPs
  36. ⇰   Labconco Purifier Class 2 Biological Safety Cabinet (BSC) Operation SOP
  37. ⇰   New Brunswick BioFlo 3000 Bioreactor SOP
  38.  
  39. Associated Quality Control Biochemistry SOPs can be found on the Quality Control Biochemistry page.
  40.  
  41. SKILL STANDARDS
  42. Upstream Processing Skill Standards
  43.  
  44. PRESENTATIONS
  45. Technology and Opportunities in Mammalian Cell Culture (56mins)
  46. By Tim Charlebois
  47.  
  48. Humanized Pichia pastoris (46 mins)
  49. By Thomas Potgieter
  50.  
  51. Growing Products in Microalgae: From Nutraceuticals to Human Proteins (56 mins)
  52. By Miguel Oliazola
  53. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 
  54. Introduction to Biomanufacturing Master Glossary
  55.  
  56. OTHER RESOURCES:
  57. White paper on Single Use Technology in Biomanufacturing
  58. By Professor Jim DeKloe, Solano College
  59.  
  60. VIRTUAL LABS
  61. Single Use Biomanufacturing
  62. username: Your Email Address
  63. password: qcbtc
  64. By Bruce van Dyke at Quincy College
  65.  
  66.  
  67. Virtual Fermentation
  68. GFP biomanufacturing
  69. Biomanufacturing e-Learning Resources
  70. By NC Bionetwork
  71.  
  72. VIDEO
  73. Bioprocessing Part 1: Fermentation (15 mins)
  74. By NC Bionetwork

 

upstream processing
Antibody Production in Microbial Hosts

 A review of the status of expression of antibodies in microbial hosts and the recent advances in the production of aglycosylated antibodies in...

upstream processing,sop
Applikon Bioreactor Operation SOP

SOP from CHO Cell - tPA Core Production Manual 

sop,upstream processing
Applikon ez-Control Bioreactor Controller Operation

SOP developed by Montgomery County Community College   

sop,upstream processing
Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP

SOP from Escherichia coli - GFP Core Production Manual 

sop,upstream processing
Batch Culture of Recombinant tPA Secreting CHO Cells

SOP developed by Montgomery County Community College   

upstream processing,sop
Batch Culture of Recombinant tPA Secreting CHO Cells SOP

SOP from CHO Cell - tPA Core Production Manual

Load More Resources
File
Chapter 10 of Introduction to Biomanufacturing 
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File
Production Unit Overview for chapters 10-12 of Introdution to Biomanufacturing 
View File

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