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  1.  
  2. The Upstream Processing unit of the NBC2 global biomanufacturing curriculum provides an overview of key elements for operation of a biomanufacturing upstream processing production area, and will help students learn to identify critical process parameters for cell growth and protein production.
  3.  
  4. TEXTBOOK CHAPTER
  5. Chapter 10: Upstream Processing

  6. Production Unit Overview

  7.  

  1. BATCH RECORDS
  2. Batch Record: GFP Production from E. coli Upstream Processing

  3. Batch Record: HSA Production from Pichia pastoris Upstream Processing 

  4. Batch Record: tPA Production from CHO Cells Upstream Process

  5.  

  6. SOPS
  7. GFP expressing E. coli

  8. Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP

  9.  

  10. Recombinant HSA secreting P. pastoris

  11. Process Controlled Fed-Batch Fermentation of Recombinant HSA Secreting Pichia pastoris SOP

  12.  

  13. tPA secreting CHO Cells

  1. Batch Culture of Recombinant tPA Secreting CHO Cells SOP 

  2.  

  3. Quality Assurance/Quality Control

  4. Glucose Determination Assay SOP

  5. Lactate Determination Assay SOP

  1. Trypan Blue Assay SOP

  2.  

  1. Operational SOPs

  2. Bellco Spinner Flask Cleaning and Autoclaving SOP 

  3. Kodak IBI Biolyzer Operation SOP

  4. Labconco Purifier Class 2 Biological Safety Cabinet (BSC) Operation SOP

  5. New Brunswick BioFlo 3000 Bioreactor SOP

  6. Operation of Life Technologies Countess II Cell Counter SOP

  7. UP 4 Applikon ez-Control Bioreactor Operation SOP

  8.  

  9. SKILL STANDARDS
  10. Upstream Processing Skill Standards

  11.  

  12. PRESENTATIONS
  13. Technology and Opportunities in Mammalian Cell Culture (56mins)

  14. By Tim Charlebois

  15.  

  16. Humanized Pichia pastoris (46 mins)

  17. By Thomas Potgieter

  18.  

  19. Growing Products in Microalgae: From Nutraceuticals to Human Proteins (56 mins)

  20. By Miguel Oliazola

  21. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 

Introduction to Biomanufacturing Master Glossary

 

  1. OTHER RESOURCES:
  2. Virtual Labs
  1. Bioprocessing Part 1: Fermentation (15 mins)

  2. By NC Bionetwork

 

introduction,upstream processing,downstream processing
Amgen Virtual Tour of a Biomanufacturing Facility

Take a virtual tour of Amgen's biomanufacturing facilities including their cell culture, recovery and purification, and formulation/distribution...

upstream processing
Antibody Production in Microbial Hosts

 A review of the status of expression of antibodies in microbial hosts and the recent advances in the production of aglycosylated antibodies in...

upstream processing,sop
Applikon Bioreactor Operation SOP

SOP from CHO Cell - tPA Core Production Manual 

sop,upstream processing
Applikon ez-Control Bioreactor Controller Operation

SOP developed by Montgomery County Community College   

sop,upstream processing
Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP

SOP from Escherichia coli - GFP Core Production Manual 

sop,upstream processing
Batch Culture of Recombinant tPA Secreting CHO Cells

SOP developed by Montgomery County Community College   

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Chapter 10 of Introduction to Biomanufacturing 
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Production Unit Overview for chapters 10-12 of Introdution to Biomanufacturing 
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