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  1. The Upstream Processing unit of the NBC2 global biomanufacturing curriculum provides curricular material for each step of the upstream processing of a biopharmaceutical.
  2.  
  3. TEXTBOOK CHAPTER
  4. Chapter 10: Upstream Processing

  5. Production Unit Overview

  6.  

  7. Expression Systems
  8. Anti-IL8 Monoclonal Antibody production in CHO cells

  9. ⇰  mAb Upstream Process Flow Chart

  10. ⇰   Batch Record: Anti-IL-8 mAb Production from CHO DP-12 Cells
  11. ⇰   Batch Culture of anti-IL-8 Monoclonal Antibody secreting CHO DP-12 Cells SOP
  12. ⇰   Applikon ez-Control Bioreactor Operation SOP
  13. ⇰   Operation of Life Technologies Countess II Cell Counter SOP
  14. ⇰   Bellco Spinner Flask Cleaning and Autoclaving SOP 
  15. ⇰   Kodak IBI Biolyzer Operation SOP
  16. ⇰   Measurement of Glucose and Lactate Concentrations using the YSI 2900 Biochemistry Analyzer SOP
  17. ⇰   Glucose Determination Assay SOP
  18. ⇰   Lactate Determination Assay SOP
  19. ⇰   Operation of Life Technologies Countess II Cell Counter SOP
  20. ⇰   Trypan Blue Assay SOP
  21.  
  22. Tissue Plasminogen Activator, tPA, production in CHO cells
  23. ⇰   Entire CHO Cell tPA Core Production System Manual  
  24. ⇰   Batch Record: tPA Production from CHO Cells Upstream Process
  25. ⇰   Batch Culture of Recombinant tPA Secreting CHO Cells SOP
  26.  
  27. Human Serum Albumin, HSA, production in Pichia pastoris cells
  28. ⇰   Entire Pichia pastoris HSA Core Production System Manual
  29. ⇰   Batch Record: HSA Production from Pichia pastoris Upstream Processing 
  30. ⇰   Process Controlled Fed-Batch Fermentation of Recombinant HSA Secreting Pichia pastoris SOP
  31.  
  32. Green Fluorescent Protein, GFP, production in Escherichia coli cells
  33. ⇰   Entire Escherichia coli GFP Core Production System Manual
  34. ⇰   Batch Record: GFP Production from E. coli Upstream Processing
  35. ⇰   Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP
  36.  
  37. Associated Upstream Processing SOPs
  38. ⇰   Labconco Purifier Class 2 Biological Safety Cabinet (BSC) Operation SOP
  39. ⇰   New Brunswick BioFlo 3000 Bioreactor SOP
  40.  
  41. Associated Quality Control Biochemistry SOPs can be found on the Quality Control Biochemistry page.
  42.  
  43. SKILL STANDARDS
  44. Upstream Processing Skill Standards
  45.  
  46. PRESENTATIONS
  47. Technology and Opportunities in Mammalian Cell Culture (56mins)
  48. By Tim Charlebois
  49.  
  50. Humanized Pichia pastoris (46 mins)
  51. By Thomas Potgieter
  52.  
  53. Growing Products in Microalgae: From Nutraceuticals to Human Proteins (56 mins)
  54. By Miguel Oliazola
  55. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 
  56. Introduction to Biomanufacturing Master Glossary
  57.  
  58. OTHER RESOURCES:
  59. White paper on Single Use Technology in Biomanufacturing
  60. By Professor Jim DeKloe, Solano College
  61.  
  62. VIRTUAL LABS
  63. Single Use Biomanufacturing
  64. username: Your Email Address
  65. password: qcbtc
  66. By Bruce van Dyke at Quincy College
  67.  
  68. Virtual Tour of a Biomanufacturing Facility
  69. By Amgen
  70.  
  71. Virtual Fermentation
  72. GFP biomanufacturing
  73. Biomanufacturing e-Learning Resources
  74. By NC Bionetwork
  75.  
  76. VIDEO
  77. Bioprocessing Part 1: Fermentation (15 mins)
  78. By NC Bionetwork

 

introduction,upstream processing,downstream processing
Amgen Virtual Tour of a Biomanufacturing Facility

Take a virtual tour of Amgen's biomanufacturing facilities including their cell culture, recovery and purification, and formulation/distribution...

upstream processing
Antibody Production in Microbial Hosts

 A review of the status of expression of antibodies in microbial hosts and the recent advances in the production of aglycosylated antibodies in...

upstream processing,sop
Applikon Bioreactor Operation SOP

SOP from CHO Cell - tPA Core Production Manual 

sop,upstream processing
Applikon ez-Control Bioreactor Controller Operation

SOP developed by Montgomery County Community College   

sop,upstream processing
Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP

SOP from Escherichia coli - GFP Core Production Manual 

sop,upstream processing
Batch Culture of Recombinant tPA Secreting CHO Cells

SOP developed by Montgomery County Community College   

Load More Resources
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Chapter 10 of Introduction to Biomanufacturing 
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File
Production Unit Overview for chapters 10-12 of Introdution to Biomanufacturing 
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