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Standard Operating Procedures

  1. Affinity Chromatography of HSA SOP
  2. Anion Exchange Chromatography of GFP using the AKTA Pure System SOP
  3. API 20E Microbial Identification SOP
  4. API 20E Manufacture Instructions
  5. API 20E Examples of Positive & Negative Tests
  6. Applikon Bioreactor Operation SOP effective 2008
  7. Applikon Bioreactor Operation SOP effective 2013
  8. Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP
  9. Batch Culture of Recombinant tPA Secreting CHO Cells SOP  
  10. Batch Culture of Recombinant tPA Secreting CHO Cells SOP
  11. Batch Record: tPA Production from CHO Cells Upstream Process SOP
  12. Bellco Spinner Flask Cleaning and Autoclaving SOP
  13. BioLogic LP Chromatography System Operation SOP
  14. BioLogic LP HIC Chromatography of GFP SOP
  15. Biomanufacturing e-Learning Resources
  16. BioTek Elx 8080UI Automated Microplate Reader SOP
  17. Buck Scientific BLC-20P HPLC Operation SOP
  18. Buck Scientific BLC-30G HPLC Operation SOP
  19. Cation Exchange Chromatography of tPA using the AKTA Pure System SOP
  20. Characterization of GFP using BLC-30G HPLC System SOP
  21. Column Chromatography of Green Fluorescent Protein SOP
  22. Conversion of Microalgae Lipids to Biodiesel SOP
  23. DAPI  (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Nucleic Acid Fluorescent Staining SOP
  24. DAPI Staining of CHO-K1 Cells SOP
  25. Degassing a Solution by Helium Sparge SOP
  26. Denver Instrument APX 323 Balance Operation SOP
  27. Denver Instruments APX 323 Balance Calibration SOP
  28. Denver Instruments PI 403 Balance Calibration SOP
  29. Denver Instruments PI 403 Balance Operation SOP
  30. Denver Instruments SI 4002 Balance Calibration SOP
  31. Denver Instruments SI 4002 Balance Operation SOP
  32. Detection of rabbit IgGs using ELISA Immunoassay SOP
  33. End-of-Run tPA Process: Harvest, Centrifugation, pH Adjustment SOP
  34. Eppendorf Research Plus Performance Verification SOP
  35. Eppendorf Research Plus Pipette Operation and Maintenance SOP
  36. Establishing and Scaling-up a Microalgae Culture for Biofuel Production  SOP
  37. Filter Validation SOP
  38. Fisher Accu 124 Balance Calibration SOP
  39. Fisher Accu 124 Balance Operation SOP
  40. Fisher Accu 124D Balance Calibration SOP
  41. Fisher Accu 124D Balance Operation SOP
  42. Four Step Gram Stain SOP
  43. GFP Production from E. coli Upstream Processing Batch Record SOP
  44. Gilson Pipetman® Operation and Maintenance SOP
  45. Gilson Pipetteman® Performance Verification SOP
  46. Glucose Determination Assay SOP
  47. Gowning for Entry into Biomanufacturing Suite SOP
  48. Gram Stain SOP
  49. Gram Stain SOP
  50. HSA ELISA SOP
  51. HSA Production from Pichia pastoris Downstream Processing Batch Record SOP
  52. HSA Production from Pichia pastoris Upstream Processing Batch Record SOP
  53. Human tPA Activity ELISA Kit SOP
  54. Human tPA Total Antigen ELISA Kit SOP
  55. Ion Exchange Chromatography of tPA on the Biologics LP System SOP
  56. Isolation of mAb (anti IL-8) from Conditioned Medium by Protein A Affinity Chromatography on the AKTA Pure Chromatography System SOP
  57. Kodak EKTACHEM DT Pipettor SOP
  58. Kodak IBI Biolyzer Operation SOP
  59. Labconco Purifier Class 2 Biological Safety Cabinet (BSC) Operation SOP
  60. Lactate Determination Assay SOP
  61. LAL Assay Gel Clot Method SOP
  62. LAL Assay-Gel Clot Method SOP
  63. Leica DME Microscope SOP
  64. Lysine Affinity Chromatography of tPA on the AKTA Pure System SOP
  65. M Air T Millipore Air Tester SOP
  66. Microalgae Lipid Extraction and Separation by TLC for Biofuel Production SOP
  67. Millipore Pellicon XL Tangential Flow Filter SOP
  68. Millipore Tangential Flow and Diafiltration Using Pellicon XL Device SOP
  69. mini-BIOMAN: Production of Stem Cells in a Regulated Environment SOP
  70. Mycoplasma Testing SOP
  71. Myoblast to Osteoblast: Analysis of C2C12 Differentiation Using BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate / nitro blue tetrazolium) SOP
  72. NBC2 Educators Conference SOP
  73. New Brunswick BioFlo 3000 Bioreactor SOP
  74. Oakton PC 700 Bench Series pH/Conductivity/C/F Meter SOP
  75. Operation of AKTA Pure Chromatography System SOP
  76. Operation of Harvey Sterilemax Autoclave SOP
  77. Operation of Life Technologies Countess II Cell Counter SOP
  78. Operation of MetOne GT-321 Particle Counter SOP
  79. Operation of MetOne Model 229B Laser Particle Counter SOP
  80. Operation of MetOne Model 299 B Laser Particle Counter SOP
  81. Operation of NanoDrop 2000 Spectrophotometer SOP
  82. Operation ofBioRad iMark Microplate Absorbance Reader SOP
  83. Operational Qualification for Autoclave SOP
  84. Pipetman Challenge SOP
  85. Pipette Performance Verification Form SOP
  86. Pipette Performance Verification Form Pass/Fail SOP
  87. Preparation of ES-E14TG2a (E14) Stem Cell Pluripotent Media (PPM) (with a note on the preparation of Differentiation Media) SOP
  88. Process Controlled Fed-Batch Fermentation of Recombinant HSA Secreting Pichia pastoris SOP
  89. Protein is Cash Manual 2014 SOP
  90. Protein is Cash Manual 2016 SOP
  91. Protein is Cash Manual Table of Contents SOP
  92. Quantification of Caffeine in Coffee using HPLC SOP
  93. SDS-PAGE Electrophoresis of GFP SOP
  94. SDS-PAGE of Green Fluorescent Protein SOP
  95. SDS-PAGE SOP
  96. Shimadzu UV-Visible Spectrophotometer SOP
  97. Split Subculturing of C2C12 Cell Line to Setup for Differentiation SOP
  98. Split Subculturing of C2C12 Cell Line with Trypsin SOP
  99. Split Subculturing of CHO-KI Cell Line into a 24-well Plate SOP
  100. Subculturing Mouse Embryonic Stem Cell Line E14 (ES-E14TG2a)(ATCC CRL-1821) SOP
  101. Tangential Flow Filtration of HSA Process SOP
  102. tPA Activity ELISA SOP
  103. tPA Direct ELISA SOP
  104. tPA Production from CHO Cells Upstream Processing Batch Record SOP
  105. tPA Production from CHO Cells using Biologics LP System Downstream Processing Batch Record SOP
  106. Transfection of CHO-K Cells with pEGFP-Actin and pDsRed2-Mito SOP
  107. Transfer of Embryoid Bodies into Gelatin-coated Plate for DifferentiationWith and Without Retinoic Acid SOP
  108. Transfer of Mouse Embryonic Stem Cell Line E14 (ES-E14TG2a)(ATCC CRL-1821) to Non-binding Petri Plates to Form Embryoid Bodies SOP
  109. Transformation SOP
  110. Trypan Blue Assay SOP
  111. Tuttnauer 3850 ELV Autoclave Operation SOP
  112. Xcell SureLock Mini-Gel Box SOP

 

 

downstream processing,sop
Affinity Chromatography of HSA SOP

SOP from Pichia pastoris - HSA Core Production Manual  

bioman,microbiological control,sop,bioman 2013,bioman 2014,bioman 2015
API 20E Microbial Identification SOP

SOP from BIOMAN QC Microbiology workshop.

upstream processing,sop
Applikon Bioreactor Operation SOP

SOP from CHO Cell - tPA Core Production Manual 

sop,upstream processing
Applikon ez-Control Bioreactor Controller Operation

SOP developed by Montgomery County Community College   

sop,upstream processing
Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP

SOP from Escherichia coli - GFP Core Production Manual 

sop,upstream processing
Batch Culture of Recombinant tPA Secreting CHO Cells

SOP developed by Montgomery County Community College   

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