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Standard Operating Procedures

  1. Affinity Chromatography of HSA SOP
  2. Anion Exchange Chromatography of GFP using the AKTA Pure System SOP
  3. API 20E Microbial Identification SOP
  4. Applikon Bioreactor Operation SOP effective 2008
  5. Applikon Bioreactor Operation SOP effective 2013
  6. Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP
  7. Batch Culture of Recombinant tPA Secreting CHO Cells SOP 
  8. Batch Record: GFP Production from E. coli Upstream Processing
  9. Batch Record: HSA Production from Pichia pastoris Downstream Processing
  10. Batch Record: HSA Production from Pichia pastoris Upstream Processing 
  11. Batch Record: tPA Production from CHO Cells Upstream Process
  12. Batch Record: tPA Production from CHO Cells Downstream Process Biologics LP System
  13. Batch Record: tPA Production from CHO Cells AKTA Pure Chromatography Operation
  14. Bellco Spinner Flask Cleaning and Autoclaving SOP 
  15. BioLogic LP Chromatography System Operation SOP
  16. BioLogic LP HIC Chromatography of GFP SOP
  17. BioTek Elx 8080UI Automated Microplate Reader SOP
  18. Buck Scientific BLC-20P HPLC Operation SOP
  19. Buck Scientific BLC-30G HPLC Operation SOP
  20. Cation Exchange Chromatography of tPA using the AKTA Pure System SOP
  21. Characterization of GFP using BLC-30G HPLC System SOP
  22. Column Chromatography of Green Fluorescent Protein SOP
  23. Conversion of Microalgae Lipids to Biodiesel SOP
  24. DAPI  (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Nucleic Acid Fluorescent Staining SOP
  25. DAPI Staining of CHO-K1 Cells SOP
  26. Degassing a Solution by Helium Sparge SOP
  27. Denver Instruments APX 323 Balance Calibration SOP
  28. Denver Instruments APX 323 Balance Operation SOP
  29. Denver Instruments PI 403 Balance Calibration SOP
  30. Denver Instruments PI 403 Balance Operation SOP
  31. Denver Instruments SI 4002 Balance Calibration SOP
  32. Denver Instruments SI 4002 Balance Operation SOP
  33. Detection of rabbit IgGs using ELISA Immunoassay SOP
  34. End-of-Run tPA Process: Harvest, Centrifugation, pH Adjustment SOP
  35. Eppendorf Research Plus Performance Verification SOP
  36. Eppendorf Research Plus Pipette Operation and Maintenance SOP
  37. Establishing and Scaling-up a Microalgae Culture for Biofuel Production  SOP
  38. Filter Validation SOP
  39. Fisher Accu 124 Balance Calibration SOP
  40. Fisher Accu 124 Balance Operation SOP
  41. Fisher Accu 124D Balance Calibration SOP
  42. Fisher Accu 124D Balance Operation SOP
  43. Gilson Pipetman® Operation and Maintenance SOP
  44. Gilson Pipetman® Performance Verification SOP
  45. Glucose Determination Assay SOP
  46. Gowning for Entry into Biomanufacturing Suite SOP
  47. Gram Stain SOP
  48. HSA ELISA SOP 
  49. Human tPA Activity ELISA Kit SOP
  50. Human tPA Total Antigen ELISA Kit SOP
  51. Ion Exchange Chromatography of tPA on the Biologics LP System SOP
  52. Isolation of mAb (anti IL-8) from Conditioned Medium by Protein A Affinity Chromatography on-         the AKTA Pure Chromatography System SOP
  53. Kodak EKTACHEM DT Pipettor SOP
  54. Kodak IBI Biolyzer Operation SOP
  55. Labconco Purifier Class 2 Biological Safety Cabinet (BSC) Operation SOP
  56. Lactate Determination Assay SOP
  57. LAL Assay Gel Clot Method SOP
  58. Leica DME Microscope SOP
  59. Lysine Affinity Chromatography of tPA on the AKTA Pure System SOP
  60. M Air T Millipore Air Tester SOP
  61. Microalgae Lipid Extraction and Separation by TLC for Biofuel Production SOP
  62. Millipore Pellicon XL Tangential Flow Filter SOP
  63. Millipore Tangential Flow and Diafiltration Using Pellicon XL Device SOP
  64. Mycoplasma Testing SOP
  65. Myoblast to Osteoblast: Analysis of C2C12 Differentiation Using BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate / nitro blue tetrazolium) SOP
  66. New Brunswick BioFlo 3000 Bioreactor SOP
  67. Oakton PC 700 Bench Series pH/Conductivity/C/F Meter SOP
  68. Operation of AKTA Pure Chromatography System SOP
  69. Operation of Harvey Sterilemax Autoclave SOP
  70. Operation of Life Technologies Countess II Cell Counter SOP
  71. Operation of MetOne GT-321 Particle Counter SOP
  72. Operation of MetOne Model 299B Laser Particle Counter SOP
  73. Operation of NanoDrop 2000 Spectrophotometer SOP
  74. Operation of BioRad iMark Microplate Absorbance Reader SOP
  75. Operational Qualification for Autoclave SOP
  76. Pipetman Challenge SOP
  77. Pipette Performance Verification Form SOP
  78. Pipette Performance Verification Form Pass/Fail SOP
  79. Preparation of ES-E14TG2a (E14) Stem Cell Pluripotent Media (PPM) (with a note on the preparation of Differentiation Media) SOP
  80. Process Controlled Fed-Batch Fermentation of Recombinant HSA Secreting Pichia pastoris SOP
  81. Quantification of Caffeine in Coffee using HPLC SOP
  82. SDS-PAGE Electrophoresis of GFP SOP
  83. SDS-PAGE SOP
  84. Shimadzu UV-Visible Spectrophotometer SOP
  85. Split Subculturing of C2C12 Cell Line to Setup for Differentiation SOP
  86. Split Subculturing of C2C12 Cell Line with Trypsin SOP
  87. Split Subculturing of CHO-KI Cell Line into a 24-well Plate SOP
  88. Subculturing Mouse Embryonic Stem Cell Line E14 (ES-E14TG2a)(ATCC CRL-1821) SOP
  89. Tangential Flow Filtration of HSA Process SOP
  90. tPA Activity ELISA SOP
  91. tPA Direct ELISA SOP
  92. Transfection of CHO-K Cells with pEGFP-Actin and pDsRed2-Mito SOP
  93. Transfer of Embryoid Bodies into Gelatin-coated Plate for DifferentiationWith and Without Retinoic
  94.     Acid SOP
  95. Transfer of Mouse Embryonic Stem Cell Line E14 (ES-E14TG2a)(ATCC CRL-1821) to Non-binding Petri Plates to Form Embryoid Bodies SOP
  96. Transformation SOP
  97. Trypan Blue Assay SOP
  98. Tuttnauer 3850 ELV Autoclave Operation SOP
  99. Xcell SureLock Mini-Gel Box SOP

 

 

downstream processing,sop
Affinity Chromatography of HSA SOP

SOP from Pichia pastoris - HSA Core Production Manual  

bioman,microbiological control,sop,bioman 2013,bioman 2014,bioman 2015
API 20E Microbial Identification SOP

SOP from BIOMAN QC Microbiology workshop.

upstream processing,sop
Applikon Bioreactor Operation SOP

SOP from CHO Cell - tPA Core Production Manual 

sop,upstream processing
Applikon ez-Control Bioreactor Controller Operation

SOP developed by Montgomery County Community College   

sop,upstream processing
Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP

SOP from Escherichia coli - GFP Core Production Manual 

sop,upstream processing
Batch Culture of Recombinant tPA Secreting CHO Cells

SOP developed by Montgomery County Community College   

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