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Standard Operating Procedures

  1. Affinity Chromatography of HSA SOP
  2. Anion Exchange Chromatography of GFP using the AKTA Pure System SOP
  3. API 20E Microbial Identification SOP
  4. API 20E Manufacture Instructions 
  5. API 20E Examples of Positive & Negative Tests
  6. Applikon Bioreactor Operation SOP effective 2008
  7. Applikon Bioreactor Operation SOP effective 2013
  8. Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP
  9. Batch Culture of Recombinant tPA Secreting CHO Cells SOP  
  10. Batch Culture of Recombinant tPA Secreting CHO Cells SOP 
  11. Batch Record: GFP Production from E. coli Upstream Processing
  12. Batch Record: HSA Production from Pichia pastoris Downstream Processing
  13. Batch Record: HSA Production from Pichia pastoris Upstream Processing 
  14. Batch Record: tPA Production from CHO Cells Upstream Process
  15. Batch Record: tPA Production from CHO Cells Downstream Process Biologics LP System
  16. Batch Record: tPA Production from CHO Cells AKTA Pure Chromatography Operation
  17. Bellco Spinner Flask Cleaning and Autoclaving SOP 
  18. BioLogic LP Chromatography System Operation SOP
  19. BioLogic LP HIC Chromatography of GFP SOP
  20. Biomanufacturing e-Learning Resources
  21. BioTek Elx 8080UI Automated Microplate Reader SOP
  22. Buck Scientific BLC-20P HPLC Operation SOP
  23. Buck Scientific BLC-30G HPLC Operation SOP
  24. Cation Exchange Chromatography of tPA using the AKTA Pure System SOP
  25. Characterization of GFP using BLC-30G HPLC System SOP
  26. Column Chromatography of Green Fluorescent Protein SOP
  27. Conversion of Microalgae Lipids to Biodiesel SOP
  28. DAPI  (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Nucleic Acid Fluorescent Staining SOP
  29. DAPI Staining of CHO-K1 Cells SOP
  30. Degassing a Solution by Helium Sparge SOP
  31. Denver Instruments APX 323 Balance Calibration SOP
  32. Denver Instruments APX 323 Balance Operation SOP
  33. Denver Instruments PI 403 Balance Calibration SOP
  34. Denver Instruments PI 403 Balance Operation SOP
  35. Denver Instruments SI 4002 Balance Calibration SOP
  36. Denver Instruments SI 4002 Balance Operation SOP
  37. Detection of rabbit IgGs using ELISA Immunoassay SOP
  38. End-of-Run tPA Process: Harvest, Centrifugation, pH Adjustment SOP
  39. Eppendorf Research Plus Performance Verification SOP
  40. Eppendorf Research Plus Pipette Operation and Maintenance SOP
  41. Establishing and Scaling-up a Microalgae Culture for Biofuel Production  SOP
  42. Filter Validation SOP
  43. Fisher Accu 124 Balance Calibration SOP
  44. Fisher Accu 124 Balance Operation SOP
  45. Fisher Accu 124D Balance Calibration SOP
  46. Fisher Accu 124D Balance Operation SOP
  47. Four Step Gram Stain SOP
  48. Gilson Pipetman® Operation and Maintenance SOP
  49. Gilson Pipetman® Performance Verification SOP
  50. Glucose Determination Assay SOP
  51. Gowning for Entry into Biomanufacturing Suite SOP
  52. Gram Stain SOP
  53. HSA ELISA SOP 
  54. Human tPA Activity ELISA Kit SOP
  55. Human tPA Total Antigen ELISA Kit SOP
  56. Ion Exchange Chromatography of tPA on the Biologics LP System SOP
  57. Isolation of mAb (anti IL-8) from Conditioned Medium by Protein A Affinity Chromatography on-         the AKTA Pure Chromatography System SOP
  58. Kodak EKTACHEM DT Pipettor SOP
  59. Kodak IBI Biolyzer Operation SOP
  60. Labconco Purifier Class 2 Biological Safety Cabinet (BSC) Operation SOP
  61. Lactate Determination Assay SOP
  62. LAL Assay Gel Clot Method SOP
  63. Leica DME Microscope SOP
  64. Lysine Affinity Chromatography of tPA on the AKTA Pure System SOP
  65. M Air T Millipore Air Tester SOP
  66. Microalgae Lipid Extraction and Separation by TLC for Biofuel Production SOP
  67. Millipore Pellicon XL Tangential Flow Filter SOP
  68. Millipore Tangential Flow and Diafiltration Using Pellicon XL Device SOP
  69. Mycoplasma Testing SOP
  70. Myoblast to Osteoblast: Analysis of C2C12 Differentiation Using BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate / nitro blue tetrazolium) SOP
  71. New Brunswick BioFlo 3000 Bioreactor SOP
  72. Oakton PC 700 Bench Series pH/Conductivity/C/F Meter SOP
  73. Operation of AKTA Pure Chromatography System SOP
  74. Operation of Harvey Sterilemax Autoclave SOP
  75. Operation of Life Technologies Countess II Cell Counter SOP
  76. Operation of MetOne GT-321 Particle Counter SOP
  77. Operation of MetOne Model 229B Laser Particle Counter SOP
  78. Operation of MetOne Model 299 B Laser Particle Counter SOP
  79. Operation of NanoDrop 2000 Spectrophotometer SOP
  80. Operation of BioRad iMark Microplate Absorbance Reader SOP
  81. Operational Qualification for Autoclave SOP
  82. Pipetman Challenge SOP
  83. Pipette Performance Verification Form SOP
  84. Pipette Performance Verification Form Pass/Fail SOP
  85. Preparation of ES-E14TG2a (E14) Stem Cell Pluripotent Media (PPM) (with a note on the preparation of Differentiation Media) SOP
  86. Process Controlled Fed-Batch Fermentation of Recombinant HSA Secreting Pichia pastoris SOP
  87. Quantification of Caffeine in Coffee using HPLC SOP
  88. SDS-PAGE Electrophoresis of GFP SOP
  89. SDS-PAGE of Green Fluorescent Protein SOP
  90. SDS-PAGE SOP
  91. Shimadzu UV-Visible Spectrophotometer SOP
  92. Split Subculturing of C2C12 Cell Line to Setup for Differentiation SOP
  93. Split Subculturing of C2C12 Cell Line with Trypsin SOP
  94. Split Subculturing of CHO-KI Cell Line into a 24-well Plate SOP
  95. Subculturing Mouse Embryonic Stem Cell Line E14 (ES-E14TG2a)(ATCC CRL-1821) SOP
  96. Tangential Flow Filtration of HSA Process SOP
  97. tPA Activity ELISA SOP
  98. tPA Direct ELISA SOP
  99. Transfection of CHO-K Cells with pEGFP-Actin and pDsRed2-Mito SOP
  100. Transfer of Embryoid Bodies into Gelatin-coated Plate for DifferentiationWith and Without Retinoic
  101.     Acid SOP
  102. Transfer of Mouse Embryonic Stem Cell Line E14 (ES-E14TG2a)(ATCC CRL-1821) to Non-binding Petri Plates to Form Embryoid Bodies SOP
  103. Transformation SOP
  104. Trypan Blue Assay SOP
  105. Tuttnauer 3850 ELV Autoclave Operation SOP
  106. Xcell SureLock Mini-Gel Box SOP

 

 

downstream processing,sop
Affinity Chromatography of HSA SOP

SOP from Pichia pastoris - HSA Core Production Manual  

bioman,microbiological control,sop,bioman 2013,bioman 2014,bioman 2015
API 20E Microbial Identification SOP

SOP from BIOMAN QC Microbiology workshop.

upstream processing,sop
Applikon Bioreactor Operation SOP

SOP from CHO Cell - tPA Core Production Manual 

sop,upstream processing
Applikon ez-Control Bioreactor Controller Operation

SOP developed by Montgomery County Community College   

sop,upstream processing
Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP

SOP from Escherichia coli - GFP Core Production Manual 

sop,upstream processing
Batch Culture of Recombinant tPA Secreting CHO Cells

SOP developed by Montgomery County Community College   

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