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CHO Cell - tPA Core Production Manual

  • cho tpa cover

The CHO Cell tPA Core Production System Manual includes upstream processing (cell culture) Standard Operating Procedures (SOPs) with batch record, downstream processing (purification) SOPs with batch record, and quality control biochemistry SOPs, including SDS PAGE, tPA ELISA, and a tPA activity assay.

 

Individual SOPs and batch record files are available below:
 

Batch Culture of Recombinant tPA Screting CHO Cells SOP

Applikon Bioreactor Operation SOP

Trypan Blue Assay SOP

Kodak IBI Biolyzer Operation SOP

Kodak EKTACHEM DT Pipettor SOP

tPA Production from CHO Cells Upstream Processing Batch Record

Ion Exchange Chromatography of tPA SOP

BioLogic LP Chromatography System Operation SOP

tPA Production form CHO Cells Downstream Processing Batch Record

SDS-PAGE SOP

Xcell SureLock Mini-Cell Gel Box SOP

tPA (direct) ELISA SOP

tPA (activity) ELISA SOP

BioTek Elx 8080UI Automated Microplate Reader SOP

 

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