Biomanufacturing Units
Introduction to BiomanufacturingUpstream ProcessingDownstream ProcessingBiomanufacture of Cell and Gene TherapiesBiomanufacture of Monoclonal AntibodiesQuality AssuranceQuality Control BiochemistryMicrobiological ControlProcess DevelopmentManufacture of a Drug ProductFacilitiesMetrologyValidationEnvironmental Health & SafetyOperational ExcellenceBiofuels
mini-BIOMAN Conferences
miniBIOMAN 2021:Monoclonal Antibody Production: HighTech and Low Tech ApproachesminiBIOMAN 2019: Design of Experiments for BiomanufacturingminiBIOMAN 2019: Quality in BiomanufacturingminiBIOMAN 2018: Downstream Processing of mAbsminiBIOMAN 2018: Incorporating Undergraduate Research in Process DevelopmentminiBIOMAN 2017: Manufacture of a Drug Product - Final Fill Finishmini-BIOMAN 2017: Upstream Processing- Mab Production in CHO cellsmini-BIOMAN 2017: Single Use Technology in Biomanufacturingmini-BIOMAN 2016: Stem Cell Production in a Regulated Environment
Upstream Processing
- The Upstream Processing unit of the NBC2 global biomanufacturing curriculum provides curricular material for each step of the upstream processing of a biopharmaceutical.
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TEXTBOOK CHAPTER
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Expression Systems
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Anti-IL8 Monoclonal Antibody production in CHO cells
- ⇰ Batch Record: Anti-IL-8 mAb Production from CHO DP-12 Cells
- ⇰ Batch Culture of CHO DP12 Cells for Production of anti-IL-8 Monoclonal Antibody SOP
- ⇰ Applikon ez-Control Bioreactor Controller Operation SOP
- ⇰ Operation of Life Technologies Countess II Cell Counter SOP
- ⇰ Bellco Spinner Flask Cleaning and Autoclaving SOP
- ⇰ Glucose and Lactate Concentration Determination using the YSI 2900 Biochemistry Analyzer SOP
- ⇰ Glucose Determination Assay Using Spectrophotometry SOP
- ⇰ Lactate Determination Assay Using Spectrophotometry SOP
- ⇰ Trypan Blue Assay for Cell Viability Determination SOP
- (See Biomanufacture of Monoclonal Antibodies page for NISTCHO cell UP)
- Tissue Plasminogen Activator, tPA, production in CHO cells
- ⇰ Entire CHO Cell tPA Core Production System Manual
- ⇰ Batch Record: tPA Production from CHO Cells Upstream Process
- ⇰ Batch Culture of Recombinant tPA Secreting CHO Cells SOP
- Human Serum Albumin, HSA, production in Pichia pastoris cells
- ⇰ Entire Pichia pastoris HSA Core Production System Manual
- ⇰ Batch Record: HSA Production from Pichia pastoris Upstream Processing
- ⇰ Process Controlled Fed-Batch Fermentation of Recombinant HSA Secreting Pichia pastoris SOP
- Green Fluorescent Protein, GFP, production in Escherichia coli cells
- ⇰ Entire Escherichia coli GFP Core Production System Manual
- ⇰ Batch Record: GFP Production from E. coli Upstream Processing
- ⇰ Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP
- Associated Upstream Processing SOPs
- ⇰ New Brunswick BioFlo 3000 Bioreactor SOP
- Associated Quality Control Biochemistry SOPs can be found on the Quality Control Biochemistry page.
- SKILL STANDARDS
- Upstream Processing Skill Standards
- SINGLE USE TECHNOLOGY
- White paper on Single Use Technology in Biomanufacturing
- By Professor Jim DeKloe, Solano College, CA
- PRESENTATIONS
- Technology and Opportunities in Mammalian Cell Culture (56mins)
- By Tim Charlebois
- Growing Products in Microalgae: From Nutraceuticals to Human Proteins (56 mins)
- By Miguel Oliazola
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- Introduction to Biomanufacturing Master Glossary
- OTHER RESOURCES:
- VIRTUAL LABS
- Single Use Biomanufacturing
- username: Your Email Address
- password: qcbtc
- By Bruce van Dyke at Quincy College
- Biomanufacturing e-Learning Resources
- By NC Bionetwork
- VIDEO
- Bioprocessing Part 1: Fermentation (15 mins)
- By NC Bionetwork
Antibody Production in Microbial Hosts
A review of the status of expression of antibodies in microbial hosts and the recent advances in the production of aglycosylated antibodies in...
Applikon Bioreactor Operation SOP
SOP from CHO Cell - tPA Core Production Manual
Applikon ez-Control Bioreactor Controller Operation
SOP developed by Montgomery County Community College
Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP
SOP from Escherichia coli - GFP Core Production Manual
Batch Culture of Recombinant tPA Secreting CHO Cells
SOP developed by Montgomery County Community College
Batch Culture of Recombinant tPA Secreting CHO Cells SOP
SOP from CHO Cell - tPA Core Production Manual
Production Unit Overview for chapters 10-12 of Introdution to Biomanufacturing
View File