Standard Operating Procedures

⇰ Affinity Chromatography of HSA SOP

⇰ AKTA Pure Operation SOP

⇰ Anion Exchange Chromatography of Green Fluorescent Protein (GFP) using the AKTA Pure System SOP

• ⇰ Anion Exchange Chromatography of Green Fluorescent Protein (GFP) using the Biologics LP System SOP
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⇰ API 20E Microbial Identification SOP

⇰ Applikon ez-Control Bioreactor Controller Operation 

⇰ Batch Culture of CHO DP12 Cells for Production of Anti IL-8 Monoclonal Antibody  SOP

⇰ CHO DP12 Spinner Flask Culture for the Production of Anti IL-8 Monoclonal Antibody SOP

⇰ Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein SOP

⇰ Batch Culture of Recombinant tPA Secreting CHO Cells SOP 

⇰ BATCH RECORD: Anti IL-8 mab Production from CHO DP-12 Cells

• ⇰ BATCH RECORD: Downstream Processing of Anti-IL8 mAb
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⇰ BATCH RECORD: GFP Production from E. coli Upstream Processing

⇰ BATCH RECORD: HSA Production from Pichia pastoris Downstream Processing

⇰ BATCH RECORD: HSA Production from Pichia pastoris Upstream Processing 

⇰ BATCH RECORD: tPA Production from CHO Cells Upstream Process

⇰ BATCH RECORD: tPA Production from CHO Cells Downstream Process Biologics LP System

⇰ BATCH RECORD: tPA Production from CHO Cells AKTA Pure Chromatography Operation

⇰ Spinner Flask Cleaning and Autoclaving SOP 

⇰ Biologic LP Chromatography System Operation SOP

⇰ BioLogic LP HIC Chromatography of GFP SOP

⇰ BioTek Elx 8080UI Automated Microplate Reader SOP

⇰ Buck Scientific BLC-20P HPLC Operation SOP

⇰ Buck Scientific BLC-30G HPLC Operation SOP

⇰ Cation Exchange Chromatography of tPA using the AKTA Pure System SOP    

⇰ Characterization of GFP using BLC-30G HPLC System SOP

⇰ CHO DP12 Spinner Flask Harvest

⇰ Column Chromatography of Green Fluorescent Protein SOP

⇰ Conversion of Microalgae Lipids to Biodiesel SOP

⇰ DAPI  (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Nucleic Acid Fluorescent Staining SOP

⇰ DAPI Staining of CHO-K1 Cells SOP

⇰ Degassing a Solution by Helium Sparge SOP

⇰ Calibration of Ohaus Pioneer Electronic Balance SOP

⇰ Denver Instruments APX 323 Balance Calibration SOP

⇰ Denver Instruments APX 323 Balance Operation SOP

⇰ Denver Instruments PI 403 Balance Calibration SOP

⇰ Denver Instruments PI 403 Balance Operation SOP

⇰ Denver Instruments SI 4002 Balance Calibration SOP

⇰ Denver Instruments SI 4002 Balance Operation SOP

⇰ Detection of rabbit IgGs using ELISA Immunoassay SOP

⇰ End-of-Run tPA Process: Harvest, Centrifugation, pH Adjustment SOP

⇰ Eppendorf Research Plus Performance Verification SOP

⇰ Eppendorf Research Plus Pipette Operation and Maintenance SOP

⇰ Establishing and Scaling-up a Microalgae Culture for Biofuel Production  SOP

⇰ Filter Validation SOP

⇰ Fisher Accu 124 Balance Calibration SOP

⇰ Fisher Accu 124 Balance Operation SOP

⇰ Fisher Accu 124D Balance Calibration SOP

⇰ Fisher Accu 124D Balance Operation SOP

⇰ Gilson Pipetman® Operation and Maintenance SOP

⇰ Gilson Pipetman® Performance Verification SOP

⇰ Glucose Determination Assay Using Spectrophotometry SOP

⇰ Glucose and Lactate Concentration determination using the YSI 2900 Biochemistry Analyzer SOP

⇰ Gowning for Entry into Biomanufacturing Suite SOP

⇰ Gram Stain SOP

⇰ HSA ELISA SOP 

⇰ Human tPA Activity ELISA Kit SOP

⇰ tPA Total Antigen ELISA Kit SOP

⇰ IL-8 Western Blot using iBLOT Dry Blotting System SOP

⇰ Ion Exchange Chromatography of tPA on the Biologics LP System SOP

• ⇰ Isolation of Anti-IL8 mAb by Protein A Affinity Chromatography on the Bio-Rad Biologic LP Chromatography System SOP
•  

⇰ Isolation of mAb (anti IL-8) from Conditioned Media by Protein A Affinity Chromatography on the AKTA Pure System 

⇰ Purification of Anti IL-8 Monoclonal Antibody from CHO DP12 Conditioned Media using Gravity Flow Protein A Chromatography

⇰ Kodak EKTACHEM DT Pipettor SOP

⇰ Kodak IBI Biolyzer Operation SOP

⇰ Labconco Purifier Class 2 Biological Safety Cabinet (BSC) Operation SOP

⇰ Lactate Determination Assay Using Spectrophotometry SOP

⇰ LAL Assay Gel Clot Method SOP

⇰ Leica DME Microscope SOP

⇰ Lysine Affinity Chromatography of tPA on the AKTA Pure System SOP

⇰ M Air T Millipore Air Tester SOP

⇰ Microalgae Lipid Extraction and Separation by TLC for Biofuel Production SOP

⇰ Millipore Pellicon XL Tangential Flow Filter SOP

⇰ Millipore Tangential Flow and Diafiltration Using Pellicon XL Device SOP

⇰ Monoclonal Aggregate Testing by HPLC Analysis SOP

⇰ Mycoplasma Testing SOP

⇰ Myoblast to Osteoblast: Analysis of C2C12 Differentiation Using BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate / nitro blue tetrazolium) SOP

⇰ New Brunswick BioFlo 3000 Bioreactor SOP

⇰ Oakton PC 700 Bench Series pH/Conductivity/C/F Meter SOP

⇰ Operation of AKTA Pure Chromatography System SOP

⇰ Operation of Harvey Sterilemax Autoclave SOP

⇰ Operation of Life Technologies Countess II Cell Counter SOP

⇰ Operation of Logos Biosystems Luna-FL Fluorescence Cell Counter for Fluorescence Cell Counting

⇰ Operation of MetOne GT-321 Particle Counter SOP

⇰ Operation of MetOne Model 299B Laser Particle Counter SOP

⇰ Operation of NanoDrop 2000 Spectrophotometer SOP

⇰ Operation of BioRad iMark Microplate Absorbance Reader SOP

⇰ Operational Qualification for Autoclave SOP

⇰ Pipetman Challenge SOP

⇰ Pipette Performance Verification Form SOP

⇰ Pipette Performance Verification Form Pass/Fail SOP

⇰ Preparation of ES-E14TG2a (E14) Stem Cell Pluripotent Media (PPM) (with a note on the preparation of Differentiation Media) SOP

⇰ Process Controlled Fed-Batch Fermentation of Recombinant HSA Secreting Pichia pastoris SOP

⇰ Quantification of Caffeine in Coffee using HPLC SOP

⇰ SDS-PAGE Electrophoresis of GFP SOP

⇰ SDS-PAGE Protein Gel Electrophoresis SOP

⇰ Shimadzu UV-Visible Spectrophotometer SOP

⇰ Split Subculturing of C2C12 Cell Line to Setup for Differentiation SOP

⇰ Split Subculturing of C2C12 Cell Line with Trypsin SOP

⇰ Split Subculturing of CHO-KI Cell Line into a 24-well Plate SOP

⇰ Subculturing Mouse Embryonic Stem Cell Line E14 (ES-E14TG2a)(ATCC CRL-1821) SOP

⇰ Tangential Flow Filtration of HSA Process SOP

⇰ tPA Activity ELISA SOP

⇰ tPA Direct ELISA SOP

⇰ Transfection of CHO-K Cells with pEGFP-Actin and pDsRed2-Mito SOP

⇰ Transfer of Embryoid Bodies into Gelatin-coated Plate for Differentiation With and Without Retinoic Acid SOP

⇰ Transfer of Mouse Embryonic Stem Cell Line E14 (ES-E14TG2a)(ATCC CRL-1821) to Non-binding Petri Plates to Form Embryoid Bodies SOP

⇰ Trypan Blue Assay for Cell Viability Determination  SOP

⇰ Tuttnauer 3850 ELV Autoclave Operation SOP

• ⇰  Viral Gene Delivery and Evaluation Using Flow Cytometry
•  

⇰ Xcell SureLock Mini-Gel Box SOP