The Pichia pastoris Core Production System Manual includes upstream processing Standard Operating Procedures (SOPs) and batch record for the process controlled fed batch fermentation of recombinant Pichia pastoris. Downstream processing SOPs include tangential flow filtration and affinity chromatography using the Biologic LP system. Quality control SOPs and a competencies list for downstream process technicians are also included.
Process Controlled Fed-Batch Fermentation of Recombinant HSA Screting Pichia pastoris SOP
New Brunswick BioFlo 3000 Bioreactor SOP
HSA Production from Pichia pastoris Upstream Processing Batch Record
Tangential Flow Filtration of HSA Process SOP
Millipore Pellicon XL Tangential Flow Filer SOP
Affinity Chromatography of HSA SOP
BioLogic LP Chromatography System Operation SOP
HSA Production from Pichia pastoris Downstream Processing Batch Record
Xcell SureLock Mini-Cell Gel Box SOP
BioTek 8080UI Automated Microplate Reader SOP
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